全文获取类型
收费全文 | 602篇 |
免费 | 94篇 |
出版年
2021年 | 10篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 12篇 |
2017年 | 8篇 |
2016年 | 12篇 |
2015年 | 28篇 |
2014年 | 28篇 |
2013年 | 27篇 |
2012年 | 42篇 |
2011年 | 50篇 |
2010年 | 26篇 |
2009年 | 21篇 |
2008年 | 23篇 |
2007年 | 27篇 |
2006年 | 29篇 |
2005年 | 20篇 |
2004年 | 35篇 |
2003年 | 23篇 |
2002年 | 35篇 |
2001年 | 11篇 |
2000年 | 14篇 |
1999年 | 15篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 6篇 |
1993年 | 4篇 |
1992年 | 16篇 |
1991年 | 17篇 |
1990年 | 13篇 |
1989年 | 14篇 |
1988年 | 14篇 |
1987年 | 13篇 |
1986年 | 6篇 |
1985年 | 10篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 7篇 |
1974年 | 5篇 |
1973年 | 2篇 |
1972年 | 4篇 |
排序方式: 共有696条查询结果,搜索用时 15 毫秒
691.
Shlomit Radom-Aizik Frank Zaldivar Szu-Yun Leu Pietro Galassetti Dan M Cooper 《Journal of applied physiology》2008,104(1):236-243
Relatively brief bouts of exercise alter gene expression in peripheral blood mononuclear cells (PBMCs), but whether exercise changes gene expression in circulating neutrophils (whose numbers, like PBMCs, increase) is not known. We hypothesized that exercise would activate neutrophil genes involved in apoptosis, inflammation, and cell growth and repair, since these functions in leukocytes are known to be influenced by exercise. Blood was sampled before and immediately after 30 min of constant, heavy ( approximately 80% peak O(2) uptake) cycle ergometer exercise in 12 healthy men (19-29 yr old) of average fitness. Neutrophils were isolated using density gradients; RNA was hybridized to Affymetrix U133+2 Genechip arrays. With false discovery rate (FDR) <0.05 with 95% confidence, a total of 526 genes were differentially expressed between before and after exercise. Three hundred and sixteen genes had higher expression after exercise. The Jak/STAT pathway, known to inhibit apoptosis, was significantly activated (EASE score, P < 0.005), but 14 genes were altered in a way likely to accelerate apoptosis as well. Similarly, both proinflammatory (e.g., IL-32, TNFSF8, and CCR5) and anti-inflammatory (e.g., ANXA1) were affected. Growth and repair genes like AREG and FGF2 receptor genes (involved in angiogenesis) were also activated. Finally, a number of neutrophil genes known to be involved in pathological conditions like asthma and arthritis were altered by exercise, suggesting novel links between physical activity and disease or its prevention. In summary, brief heavy exercise leads to a previously unknown substantial and significant alteration in neutrophil gene expression. 相似文献
692.
Deltamethrin (RU 22974) and tralomethrin isomers (RU 24784 and RU 24785) block transmission at the cercal-afferent giant-interneuron synapses of the cockroach, when bath-applied to the desheathed ganglion at micromolar concentrations. The time-course of the events leading to the blockage suggests two possible target sites: one located presynaptically and the other situated on postsynaptic membranes. 相似文献
693.
Jui‐Yu Chou Jun‐Yi Leu 《BioEssays : news and reviews in molecular, cellular and developmental biology》2010,32(5):401-411
Several features of the yeast mitochondrial genome, including high mutation rate, dynamic genomic structure, small effective population size, and dispensability for cellular viability, make it a promising candidate for generating hybrid incompatibility and driving speciation. Cytonuclear incompatibility, a specific type of Dobzhansky‐Muller genetic incompatibility caused by improper interactions between mitochondrial and nuclear genomes, has previously been observed in a variety of organisms, yet its role in speciation remains obscure. Recent studies in Saccharomyces yeast species provide a new insight, with experimental evidence that cytonuclear incompatibility and DNA sequence divergence are both causes of the reproductive isolation of different yeast species. Interestingly, these two mechanisms seem to be perfectly complementary to each other in terms of their effects and evolutionary trajectories. Direct molecular analyses of the incompatible genes in yeasts have started to shed light on the evolutionary forces driving speciation. Editor's suggested further reading in BioEssays The cytoplasmic structure hypothesis for ribosome assembly, vertical inheritance, and phylogeny Abstract Mitochondrial bioenergetics as a major motive force of speciation Abstract 相似文献
694.
Fructose 2,6-bisphosphate in isolated rat enterocytes. 总被引:1,自引:1,他引:0
695.
696.
W W Sheng S Y Leu K D Wuu 《Proceedings of the National Science Council, Republic of China. Part B, Life sciences》1991,15(2):111-116
A chromosomal analysis was carried out on two colorectal carcinoma cell lines (WiDr and COLO 205), which were established 15-20 years ago in the US and were collected by the Cell Bank of the Veterans General Hospital in Taipei. Among the 200 cells counted, 65.5% of WiDr (male) had 68-73 chromosomes, while 74% of the COLO 205 (female) had 70-76 chromosomes per cell. The Y chromosome was absent in the 30 WiDr metaphases analyzed. None of the other chromosomes, including X and the autosomes of both WiDr and COLO 205, revealed such a numerical deficiency. Over half of the autosomes had an average number per cell above 2.0. The existence of 5 or 6 normal homologues for certain autosomes was not rare in either line. Numerous structural abnormal marker chromosomes were present in the cells. As compared with the original chromosome findings which were done over 10 years ago, we noted that the range of chromosome counts was wider and the number of marker chromosomes increased in these long-term cultivated cell lines. 相似文献